NBR1 | Autophagy substrate NBR1 (affinity purified)

Inquiry

Cat: PA00542

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

UNA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 500-1 : 5000 (WB)

Expected | apparent MW

75 | 100 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Brassicaceae family, Solanum lycopersicum

Not reactive in

Nicotiana tabacum

Additional information

A specific extraction method and tissue type needs to be used as described in Minina et al, (2013), Dilution in western blot depends upon amount of NBR1 in the sample

Description

NBR1 (Autophagy substrate NBR1) is involved in selective autophagy of damaged organelles, intracellular microorganisms, protein aggregates, cell structures, and specific soluble proteins. NBR1 mediates this process as an autophagy adapter. The protein has two UBA domains, but only the C-terminal UBA domain binds to ubiquitin. Alias :At4g24690, presumably uncharacterized protein F22K18.110.

For research use only, not for clinical use.