Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Biotinylated

NPL, isolated from daffodil bulbs, is specific for alpha-linked mannose, preferring polymannose structures containing (alpha-1,6) bonds. Binding to the mannose polymer can occur through internal mannose residues and does not depend on the structural integrity of the non-reducing end sugars. NPL also binds some galactomannan and differs in other binding properties from the related lectin Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose. Biotinated daffodil lectin binds the appropriate amount of biotin to provide the best staining properties for this lectin. This conjugate contains essentially no unconjugated biotin and is preserved with sodium azide.