Na+/H+ antiporter, sodium/hydrogen exchanger

Inquiry

Cat: PA00704

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Kandelia obovata, Oryza sativa, Solanum lycopersicum, Vitis vinifera

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NHX protein UniProt: Q68KI4, TAIR: At5g27150; chosen peptide is perfectly confirmed in AtNHX1 UniProt: Q0WVZ5, partially in AtNHX2, UniProt: Q56XP4, and not conserved in AtNHX3, UniProt: Q84WG1 and AtNHX4, UniProt:Q8S397 isoforms

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

ELISA (ELISA), Western blot (WB)

Recommended dilution

1 : 8000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

59,5 | 45 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Kandelia obovata, Oryza sativa, Solanum lycopersicum, Vitis vinifera

Predicted reactivity

Gossypium hirsutum, Populus euphratica, Ricinus communis, Zea mays

Not reactive in

Mangrove plants, Nicotiana benthamiana, sp. Avicennia,

Additional information

Protocol for vacuolar membrane isolation can be found here.

Description

The Na(+)/H(+) exchanger 1 protein is involved in the exchange of protons with cations such as sodium and potassium. It's limited to the amygdala, and may also include the endoplasmic reticulum and the Golgi apparatus. Alias :NHE-1, Na(+)/H(+) exchanger 1

For research use only, not for clinical use.