NADP-dependent alkenal double bond reductase P1 (P1) (AER), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana NADP-dependent alkenal double bond reductase P1 (P1)

Product Gene Name

AER recombinant protein

Product Synonym Gene Name

P1

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MTATNKQVIL KDYVSGFPTE SDFDFTTTTV ELRVPEGTNS VLVKNLYLSC DPYMRIRMGK PDPSTAALAQ AYTPGQPIQG YGVSRIIESG HPDYKKGDLL WGIVAWEEYS VITPMTHAHF KIQHTDVPLS YYTGLLGMPG MTAYAGFYEV CSPKEGETVY VSAASGAVGQ LVGQLAKMMG CYVVGSAGSK EKVDLLKTKF GFDDAFNYKE ESDLTAALKR CFPNGIDIYF ENVGGKMLDA VLVNMNMHGR IAVCGMISQY NLENQEGVHN LSNIIYKRIR IQGFVVSDFY DKYSKFLEFV LPHIREGKIT YVEDVADGLE KAPEALVGLF HGKNVGKQVV VVARE

Sequence Positions

1-345aa; Full Length

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

38.1 kDa

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Aerotaxis receptor

NCBI Accession #

NP_197199.1

NCBI GI #

15237888

NCBI GenBank Nucleotide #

NM_121703.4

NCBI GeneID

831560

NCBI Official Full Name

alkenal reductase

NCBI Official Symbol

AER

NCBI Official Synonym Symbols

alkenal reductase; AT-AER; F2K13.120; F2K13_120

NCBI Protein Information

alkenal reductase

NCBI Summary

encodes a 2-alkenal reductase (EC 1.3.1.74), plays a key role in the detoxification of reactive carbonyls

UniProt Gene Name

AER

UniProt Synonym Gene Names

P1-ZCr

UniProt Protein Name

NADPH-dependent oxidoreductase 2-alkenal reductase

UniProt Synonym Protein Names

NADP-dependent alkenal double bond reductase P1

UniProt Primary Accession #

Q39172

UniProt Secondary Accession #

Q501A9; Q8L865

UniProt Related Accession #

Q39172

UniProt Comments

Involved in the detoxification of reactive carbonyls (PubMed:10848984, PubMed:12514241, PubMed:16299173). Acts on lipid peroxide-derived reactive aldehydes (PubMed:12514241). Specific to a double bond activated by an adjacent carbonyl group (PubMed:12514241). Can use both quinones and diamide as substrates, but not menadione, ferricyanide or phylloquinone (PubMed:10848984). Can use 4-hydroxy-(2E)-nonenal (HNE), 4-hydroxy-(2E)-hexenal (HHE), (2E)-nonenal, (2E)-hexenal, (2E)-pentenal, propenal (acrolein), 3-buten-2-one and 3-penten-2-one, but not (R)---carvone, n-nonanal, n-hexanal, (3Z)-hexanal, cyclohex-2-en-1-one or 12-oxo phytodienoic acid (OPDA) as electron acceptors (PubMed:12514241). Catalyzes the reduction of the alpha,beta-unsaturated bond of 2-alkenals, of lipid peroxide-derived oxenes 9-oxo-10(E),12(Z)-octadecadienoic acid (9-KODE) and 13-oxo-9(Z),11(E)-octadecadienoic acid (13-KODE), as well as 4-oxo-(2E)-nonenal and 4-hydroxynonenal (PubMed:16299173). Can use 12-oxo-10(E) dodecanoate (traumatin), trans-1,3 diphenyl-2-propenone, trans-1,4-diphenyl-2-butene-1,4-dione, 9-oxo-12,13-epoxy-(10E)-octadecenoic acid (trans-EKODE-1b) and 9,13-dihydroxy-10-oxo-11-octadecenoic acid as substrates (PubMed:26678323). Catalyzes the reduction of the 7-8 double bond of phenylpropanal substrates, such as p-coumaryl aldehyde and coniferyl aldehyde (in vitro) (PubMed:17028190). Has activity towards toxic substrates, such as 4-hydroxy-(2E)-nonenal (in vitro) (PubMed:17028190). May play a distinct role in plant antioxidant defense and is possibly involved in NAD(P)/NAD(P)H homeostasis (PubMed:17028190).