NAD-ME | Mitochondrial NAD-dependent malic enzyme
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NAD-ME | Mitochondrial NAD-dependent malic enzyme

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Cat: PA01098
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Nicotiana tabacum, Zea mays
Datasheet:

Product Info

Immunogen
Native, NAD-ME purified from mitochondria isolated from mature amaranth leaves, UniProt: P37224
Host
Rabbit
Clonality
Polyclonal
Purity
Serum + 0.01% sodium azide.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunoprecipiatation (IP), Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 25 to 1 : 100 (IL), 1 : 1000 (WB)
Expected | apparent MW
65 kDa

Reactivity

Confirmed reactivity
Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Nicotiana tabacum, Zea mays
Predicted reactivity
Amborella trichopoda, Arabis alpina, Beta vulgaris, Brachypodium distachyon, Brassica oleracea, Citrus sinensis, Coffea canephora, Cucumis sativus , Cynara cardunculus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium raimondii, Hordeum vulgare, Jatropha curcas, Kalanchoe fedtschenkoi, Malus domestica, Medicago truncatula, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Physcomitrium patens, Picea sitcHensis, Populus trichocarpa, Prunus persica, Ricinus communis, Selaginella moellendorffii, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zostera marina, Wollemia nobilis, Vitis vinifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody can be used in immunolocalization using immunogold TEM.This antibody recognizes the large subunit (MEL) of the NAD-ME.Immunolocalization method is described in Long et al. (1994).

Description

nadd-me (Mitochondrial NADD-dependent malidase) is an enzyme that catalyzes the decarboxylation of 4-malate carbon in bunch-sheath cells and releases carbon dioxide in some C4 plants. This enzyme plays a key role in carbon fixation during photosynthesis.

For research use only, not for clinical use.