MnSOD3 | Superoxide dismutase (Algal)

Inquiry

Cat: PA01527

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Chlamydomonas reinhardtii

Datasheet:

Product Info

Immunogen

Recombinant MnSOD3 of Chlamydomonas reinhardtii, product of a MSD3 gene Cre16.g676150; Phytozome

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1: 1000 (WB)

Expected | apparent MW

32 | 35 kDa

Reactivity

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

green algae

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Specific extraction method requires to be applied, check as published in Page et al. (2012).MnSOD3 can be only detected in Fe-limited cells (0.5 or 0.2 mM added Fe) (i.e., samples exhibiting the novel MnSOD activity) but not in cells grown with higher concentrations of added Fe.

Description

MnSOD3(superoxide dismutase) is a chloroplast enzyme that destroys free radicals produced within cells, which are toxic to biological systems. Page et al.(2012) showed that it is induced under Fe restriction, Mn deficiency and H2O2 stress.

For research use only, not for clinical use.