MIGS5.1 (FF572)

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Cat: PP00010

Vector type: Plant Expression

Datasheet:

Product Info

Vector backbone

pGreenII

Modifications to backbone

1) Addition of an expression cassette with the miR173, however with no promoter, this should be added by multi-site gateway;
2) No promoter for expression of the MIGS construct. Instead, a promoter of choice can be cloned using multi-site gateway. See original article: de felippes et all, 2012, the Plant Journal.

Selectable markers

Basta

Bacterial Resistance(s)

Chloramphenicol and Spectinomycin, 25 & 50 μg/mL

Growth Temperature

37℃

Growth Strain(s)

ccdB Survival

Growth instructions

1) Before recombination Chloramphenicol might be used for bacteria selection (resistance lost after recombination). 2) E. coli DB3.1 strain (or some other ccdB survival strain) should be used for the amplification of the plasmid due to the presence of the lethal ccdB gene. After recombination, any common strain, such as DH5α is recommended. 3) Due to pGreen backbone characteristics, the helper plasmid pSOUP is required for replication of MIGS vector in Agrobacterium.

Copy number

High Copy

For research use only, not for clinical use.