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MIGS4.2 (FF575)
Cat: PP00009
Vector type: Plant Expression
Datasheet:
Product Info
Vector backbone
pGreenII
Modifications to backbone
1) Addition of an expression cassette with the miR173 precursor placed behind the UBQ11 constitutive promoter;
2) No promoter for expression of the MIGS construct. Instead, a promoter of choice can be cloned using the MCS just upstream of the attR1 site
2) No promoter for expression of the MIGS construct. Instead, a promoter of choice can be cloned using the MCS just upstream of the attR1 site
Selectable markers
kanamycin
Bacterial Resistance(s)
Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
Growth Temperature
37℃
Growth Strain(s)
ccdB Survival
Growth instructions
1) Before recombination Chloramphenicol might be used for bacteria selection (resistance lost after recombination). 2) E. coli DB3.1 strain (or some other ccdB survival strain) should be used for the amplification of the plasmid due to the presence of the lethal ccdB gene. After recombination, any common strain, such as DH5α is recommended. 3) Due to pGreen backbone characteristics, the helper plasmid pSOUP is required for replication of MIGS vector in Agrobacterium.
Copy number
High Copy
For research use only, not for clinical use.