mCherry (rabbit antibody)

Inquiry

Cat: PA00060

Size: 100 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: mCherry

Datasheet:

Product Info

Immunogen

Recombinant full length mCherry expressed and purified from E. coli.

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified in PBS pH 7.4, with 3% trehalose.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunocytochemistry (ICC), Western blot (WB)

Recommended dilution

1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB)

Reactivity

Confirmed reactivity

mCherry

Predicted reactivity

mScarlett

Additional information

In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.Sequence identity between mCherry and original GFP protein is 27%. Antibody does not cross with GFP or eGFP and likely not any of the relatives in western blot.

Description

mCherry is derived from DsRed, a red fluorescent protein from the genus Discoid. DsRed is a 223-amino acid ~28kDa protein that is similar in size and properties to GFP, but it produces a red rather than a green fluorescent dye. The monomer form of mCherry is useful for applications such as Forster Resonance energy transfer (FRET, also known as fluorescence resonance energy transfer). The protein is an engineered derivative of one of the protein families originally isolated from cnidarians (jellyfish, anemones, and corals).

For research use only, not for clinical use.