Magnesium-chelatase subunit chlI, chloroplastic (CHLI), Recombinant Protein
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Magnesium-chelatase subunit chlI, chloroplastic (CHLI), Recombinant Protein

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Cat: RP01020
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Magnesium-chelatase subunit chlI, chloroplastic (CHLI)
Product Gene Name
CHLI recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
VMNVATEINS TEQVVGKFDS KKSARPVYPF AAIVGQDEMK LCLLLNVIDP KIGGVMIMGD RGTGKSTTVR SLVDLLPEIN VVAGDPYNSD PIDPEFMGVE VRERVEKGEQ VPVIATKINM VDLPLGATED RVCGTIDIEK ALTEGVKAFE PGLLAKANRG ILYVDEVNLL DDHLVDVLLD SAASGWNTVE REGISISHPA RFILIGSGNP EEGELRPQLL DRFGMHAQVG TVRDADLRVK IVEERARFDS NPKDFRDTYK TEQDKLQDQI STARANLSSV QIDRELKVKI SRVCSELNVD GLRGDIVTNR AAKALAALKG KDRVTPDDVA TVIPNCLRHR LRKDPLESID SGVLVSEKFA EIFS
Sequence Positions
61-424, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
46,270 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Magnesium-chelatase

NCBI/Uniprot Data

NCBI Accession #
NP_193583.1
NCBI GI #
15233888
NCBI GenBank Nucleotide #
NM_117962.3
NCBI GeneID
827580
NCBI Official Full Name
P-loop containing nucleoside triphosphate hydrolases superfamily protein
NCBI Official Symbol
CHLI1
NCBI Official Synonym Symbols
CH-42; CH42; CHL11; CHLI-1; CHLORINA 42; F28J12.140; F28J12_140; LOST1; low temperature with open-stomata 1; PROTOPORPHYRIN-IX MG-CHELATASE
NCBI Protein Information
P-loop containing nucleoside triphosphate hydrolases superfamily protein
NCBI Summary
Encodes the CHLI subunit of magnesium chelatase which is required for chlorophyll biosynthesis. All four cysteine residues of the protein form two disulfide bonds (Cys102-Cys193 and Cys354-Cys396) under oxidized conditions but are fully reduced by reduction. It was suggested that the redox state of CHLI is regulated in vivo by the change of the redox environment in the chloroplasts probably via the Trx system.
UniProt Gene Name
CHLI1
UniProt Synonym Gene Names
CH42; CS; Mg-chelatase subunit I-1
UniProt Protein Name
Magnesium-chelatase subunit ChlI-1, chloroplastic
UniProt Synonym Protein Names
Mg-protoporphyrin IX chelatase subunit ChlI-1; Protein CHLORINA 42
UniProt Primary Accession #
P16127
UniProt Secondary Accession #
Q0WUK7
UniProt Related Accession #
P16127
UniProt Comments
Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. The magnesium-chelatase is a complex of three subunits, CHLI, CHLD and CHLH. The reaction takes place in two steps, with an ATP-dependent activation followed by an ATP-dependent chelation step. Possesses high affinity for ATP and may play a major role in chlorophyll biosynthesis. Does not bind abscisic acid (ABA), but is a positive regulator of ABA signaling.

For research use only, not for clinical use.