Lysine-specific demethylase REF6 (REF6), Recombinant Protein
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Lysine-specific demethylase REF6 (REF6), Recombinant Protein

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Cat: RP01362
Size: 0.05 mg (E-Coli)/ 0.05 mg (Yeast)/ 0.05 mg (Baculovirus)/ 0.2 mg (E-Coli)/ 0.5 mg (E-Coli)/ 0.2 mg (Yeast)/ 0.05 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 0.5 mg (Yeast)/ 1 mg (E-Coli)/ 0.5 mg (Baculovirus)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (B
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Lysine-specific demethylase REF6 (REF6) , partial
Product Gene Name
REF6 recombinant protein
Product Synonym Gene Name
REF6
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
LPVAPEFRPT LAEFQDPIAY ILKIEEEASR YGICKILPPL PPPSKKTSIS NLNRSLAARA AARVRDGGFG ACDYDGGPTF ATRQQQIGFC PRKQRPVQRP VWQSGEEYSF GEFEFKAKNF EKNYLKKCGK KSQLSALEIE TLYWRATVDK PFSVEYANDM PGSAFIPLSL AAARRRESGG EGGTVGETAW NMRAMSRAEG SLLKFMKEEI PGVTSPMVYV AMMFSWFAWH VEDHDLHSLN YLHMGAGKTW YGVPKDAALA FEEVVRVHGY GEELNPLVTF STLGEKTTVM SPEVFVKAGI PCCRLVQNPG EFVVTFPGAY HSGFSHGFNF GEASNIATPE WLRMAKDAAI RRAAIN
Sequence Positions
17-372. Partial
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
152,629 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Lysine-specific demethylase

NCBI/Uniprot Data

NCBI Accession #
NP_680116.2
NCBI GI #
79586698
NCBI GenBank Nucleotide #
NM_148863.4
NCBI GeneID
824002
NCBI Official Full Name
relative of early flowering 6
NCBI Official Symbol
REF6
NCBI Official Synonym Symbols
JMJ12; Jumonji domain-containing protein 12; relative of early flowering 6
NCBI Protein Information
relative of early flowering 6
NCBI Summary
Relative of Early Flowering 6 (REF6) encodes a Jumonji N/C and zinc finger domain-containing protein that acts as a positive regulator of flowering in an FLC-dependent pathway. REF6 mutants have hyperacetylation of histone H4 at the FLC locus. REF6 interacts with BES1 in a Y2H assay and in vitro. REF6 may play a role in brassinoteroid signaling by affecting histone methylation in the promoters of BR-responsive genes. It is most closely related to the JHDM3 subfamily of JmjN/C proteins.
UniProt Gene Name
REF6
UniProt Protein Name
Lysine-specific demethylase REF6
UniProt Synonym Protein Names
Jumonji domain-containing protein 12
UniProt Primary Accession #
Q9STM3
UniProt Related Accession #
Q9STM3
UniProt Comments
Histone demethylase that demethylates 'Lys-27' (H3K27me) of histone H3. Demethylates both tri- (H3K27me3) and di-methylated (H3K27me2) H3K27me (PubMed:21642989, PubMed:27111035). Demethylates also H3K4me3/2 and H3K36me3/2 in an in vitro assay (PubMed:20711170). Involved in the transcriptional regulation of hundreds of genes regulating developmental patterning and responses to various stimuli (PubMed:18467490). Binds DNA via its four zinc fingers in a sequence-specific manner, 5'-CTCTGYTY-3', to promote the demethylation of H3K27me3 and the regulation of organ boundary formation (PubMed:27111034, PubMed:27111035). Involved in the regulation of flowering time by repressing FLOWERING LOCUS C (FLC) expression (PubMed:15377760). Interacts with the NF-Y complexe to regulate SOC1 (PubMed:25105952). Mediates the recruitment of BRM to its target loci (PubMed:27111034).

For research use only, not for clinical use.