Immunogen
Purified Luciferase from Photinus pyralis, UniProt: P08659
Purity
Purified total IgG fraction.
Format
Lyophilized in PBS pH 7,4, no preservative added
Reconstitution
For reconstitution add 1 ml of sterile destilled water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Application
ELISA (ELISA), Dot blot (Dot), Indirect Immunofluorescence (IF), Western blot (WB)
Recommended dilution
assay dependent
Expected | apparent MW
60.74 kDa