LHY | Late elongated hypocotyl

Due to biology of LHY protein it accumulates short after dawn and not in the later day, therefore proper material has to be used to obtain a signal with this antibody. Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after adding the extraction buffer, followed by thawing directly in 100°C heating block not in RT. When the samples are heated for 10 min, spinning of the extract for 5 min is needed to remove the cell debris. From the liquid extract measurement can be done and the rest, frozen in liquid Nitrogen, in aliquots. Frozen samples will not be thawed again to 100°C, but on RT and loaded as soon as they are liquid. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.This product can be sold containing ProClin if requested

LHY (Late elongated hypocotyl) is a transcription factor involved in the biological clock. It binds to the promoter regions of APRR1/TOC1 and TCP21/CHE, inhibiting their transcription. Nicknames: Late elongation Hypocotyl, Late elongation hypocotyl 1,LHY, LHY1.