Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII
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Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII

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Cat: PA00534
Size: 0.5 mg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Cucumis sativus L. cv. Jihong no. 2, Drosera capensis, Hordeum vulgare, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Triticum aestivum, Triticale, Zea mays
Datasheet:

Product Info

Immunogen
BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At3g08940 and Lhcb4.3 At2G40100).
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 250 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 7 000 (WB)
Expected | apparent MW
31.9 | 29 kDa for Arabidopsis thaliana

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Cucumis sativus L. cv. Jihong no. 2, Drosera capensis, Hordeum vulgare, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Triticum aestivum, Triticale, Zea mays
Predicted reactivity
Catalpa bungei, Cucumis sativus, Populus, gymnosperms and microalgae Ostrecococcus tauri; the target sequence is only weakly conserved in Physcomitrium patens
Not reactive in
Chlamydomonas reinhardtii (please use AS06 117 for this organism)

Additional information

An overview about the different Lhc-protein types in plants can be found in Klimmek et al. (2006) Abundantly and rarely expressed Lhc protein genes exhibit distinct regulation patterns in plants. Plant Physiol 140: 793-804.Lhcb4 protein is processed into mature form (Jansson 1999).

Description

Lhcb4 (CP29) is one of three minor chlorophyll a/b binding proteins associated with photosystem II in plants and algae. Lhcb4 is thought to regulate the chl excited state concentration of PSII because of its ability to sense lumen pH, resulting in reversible phosphorylation. In Arabidopsis, two genes encode two subtypes, Lhcb4.1 and Lhcb4.2. A third isomer (Lhcb4.3, At2g40100), which may only be present in dicots, is found to be regulated differently and is therefore labeled Lhcb8.

For research use only, not for clinical use.