Lhcb3 | LHCII type III chlorophyll a/b-binding protein

Cat: PA00220

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Arachis hypogaea, Chlorella vulgaris, Cucumis sativa, Dactylis glomeRata, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomirella patens, Prasinoderma sp., Pyramimonas sp., Spinacia oleracea, Triticum aestivum, Triticale, Zea may, Verbascum lychnitis

Datasheet:

Product Info

Immunogen

BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb3 UniProt: Q9S7M0,TAIR:AT5G54270. This sequence is highly conserved even in Ginko biloba and one of the major LHCII-forms of Physcomitrella patens.

Host

Rabbit

Clonality

Polyclonal

Purity

Total IgG. Protein G purified in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 2000 (WB)

Expected | apparent MW

28.7 | 26 kDa for Arabidopsis thaliana

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Arachis hypogaea, Chlorella vulgaris, Cucumis sativa, Dactylis glomeRata, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomirella patens, Prasinoderma sp., Pyramimonas sp., Spinacia oleracea, Triticum aestivum, Triticale, Zea may, Verbascum lychnitis

Predicted reactivity

Cucumis melo, Dicots, Gymnosperms, Mosses

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.,Protein is processed into mature form (Jansson 1999).

Description

The main light-collecting antenna complex II (LHCII) of photosynthetic eukaryotes is located on the thylakoid membrane of the chloroplast. It is a heterotrimer complex composed of three different subtypes of chlorophyll a/b binding proteins Lhcb1, Lhcb2 and Lhcb3. While Lhcb1 and Lhcb2 are very similar and often present in multiple gene copies, the Lhcb3 protein differs in pigment composition and molecular size, and is usually encoded by only a single gene. Lhcb3 does not appear to be present in the mobile LHCII trimers involved in the state 1-state 2 transition. The molecular characterization of the LHCII protein can be found in Caffarri et al. (2004). "LHCII: Differential analysis of the Lhcb1-3 complex for Constructing the main trimer antenna complex for photosynthesis in Higher Plants". Biochemistry 43(29): 967-976.

For research use only, not for clinical use.