Lhcb2 | LHCII type II chlorophyll a/b-binding protein
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Lhcb2 | LHCII type II chlorophyll a/b-binding protein

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Cat: PA00218
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Arachis hypogaea, Brachypodium sylvaticum, Cicer arietum, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Chlamydomonas reinhardtii, Cucumis sativus, Cytisus cantabricus (Wilk.) Rchb. F., Hieracium pilosella L., Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lycopersicon esculentum (Solanum lycopersicon), Miscanthus x giganteus, Mesembryanthemum crystallinum, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus coccineus L., Phaseolus vulgaris,Physcomitrium patens, Sinapsis alba, Spinacia oleracea, Syntrichia muralis (Hedw.) Raab, Triticum aestivum, Triticale, Zea mays
Datasheet:

Product Info

Immunogen
BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb2 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb2.1 UniProt: Q9SHR7, TAIR: AT2G05100, Lhcb2.2 UniProt: Q9S7J7, TAIR:AT2G05070, Lhcb2.3 UniProt:Q9XF87, TAIR:AT3G27690
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunoprecipitation (IP), ImmunoGold (IG), Western blot (WB)
Recommended dilution
5 µl of antibody solution (IP), 1: 100 (IG), 1: 500 - 1 : 5000 (WB)
Expected | apparent MW
28.6 | 25 kDa for Arabidopsis thaliana

Reactivity

Confirmed reactivity
Acer pseudoplatanus, Arabidopsis thaliana, Arachis hypogaea, Brachypodium sylvaticum, Cicer arietum, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Chlamydomonas reinhardtii, Cucumis sativus, Cytisus cantabricus (Wilk.) Rchb. F., Hieracium pilosella L., Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lycopersicon esculentum (Solanum lycopersicon), Miscanthus x giganteus, Mesembryanthemum crystallinum, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus coccineus L., Phaseolus vulgaris,Physcomitrium patens, Sinapsis alba, Spinacia oleracea, Syntrichia muralis (Hedw.) Raab, Triticum aestivum, Triticale, Zea mays
Predicted reactivity
Algae, Dicots, Gymnosperms, Mosses
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Immunoprecipitation has been done using Immunoprecipitation kit from Roche, Cat.No. 11 719 386 001.Protein is processed into mature form (Jansson 1999).

Description

The main light-collecting antenna complex II (LHCII) of photosynthetic eukaryotes is located on the thylakoid membrane of the chloroplast. It is a heterotrimer complex composed of three different subtypes of chlorophyll a/b binding proteins Lhcb1, Lhcb2 and Lhcb3. Lhcb2 is typically encoded by several nuclear genes and, together with Lhcb1, is found in mobile LHCII trimers involved in the stat1-stat2 transition. The molecular characterization of the LHCII protein can be found in Caffarri et al. (2004). "LHCII: Differential analysis of the Lhcb1-3 complex for Constructing the main trimer antenna complex for photosynthesis in Higher Plants". Biochemistry 43(29): 967-976.

For research use only, not for clinical use.