Lhcb1 | LHCII type I chlorophyll a/b-binding protein
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Lhcb1 | LHCII type I chlorophyll a/b-binding protein

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Cat: PA00216
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Arachis hypogaea, Brassica napus, Citrus retuculata, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Rhoeo discolor, Silene vulgaris, Sinapsis alba, Solanum lycopersicum, Spinacia oleracea, Tillandsia flabellate, Triticum aestivum, Triticale, Zea mays
Datasheet:

Product Info

Immunogen
BSA-conjugated, 11 amino acidslong synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb1.1 UniProt: P0CJ48-1, TAIR: AT1G29920,   Lhcb1.2 UniProt: Q8VZ87-1, TAIR: AT1G29910, Lhcb1.3 (most expressed) UniProt: P04778-1, TAIR: AT1G2993, Lhcb1.4 UniProt: Q39142-1 , TAIR: AT2G34430, Lhcb1.5 UniProt: Q39141-1,  TAIR: At2g34420
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
28 | 25 kDa for Arabidopsis thaliana

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Arachis hypogaea, Brassica napus, Citrus retuculata, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Rhoeo discolor, Silene vulgaris, Sinapsis alba, Solanum lycopersicum, Spinacia oleracea, Tillandsia flabellate, Triticum aestivum, Triticale, Zea mays
Predicted reactivity
Aegilops tauschii, Catalpa bungei, Cucumis sativus, Brachypodium distachyon, Lotus japonicus, Hordeum vulgare, Musa acuminata, Physcomitrium patens, Solanum tuberosum, Zosteria marina, Vitis vinifera
Not reactive in
Picea abies

Additional information

Lhcb1 Protein is processed into mature form (Jansson 1999).This product can be sold containing ProClin if requested

Description

The main light-collecting antenna complex II (LHCII) of photosynthetic eukaryotes is located on the thylakoid membrane of the chloroplast. It is a heterotrimer complex composed of three different subtypes of chlorophyll a/b binding proteins Lhcb1, Lhcb2 and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b binding protein in eukaryotic phototrophs and is usually encoded by multiple nuclear genes. The molecular characterization of the LHCII protein can be found in Caffarri et al. (2004). "LHCII: Differential analysis of the Lhcb1-3 complex for Constructing the main trimer antenna complex for photosynthesis in Higher Plants". Biochemistry 43 (29): 967-976

For research use only, not for clinical use.