Lhcb1 | LHCII type I chlorophyll a/b-binding protein (Arabidopsis specific)
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Lhcb1 | LHCII type I chlorophyll a/b-binding protein (Arabidopsis specific)

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Cat: PA00560
Size: 100 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Pinus strobus L.
Datasheet:

Product Info

Immunogen
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430  (Lhcb1.4), and At2g34420 (Lhcb1.5)
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2500-1 : 5000 (WB)
Expected | apparent MW
28 | 25 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Pinus strobus L.
Predicted reactivity
Algae, Dicots, Mosses
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII:  Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476,This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004Protein is processed into mature form (Jansson 1999).

Description

The main light-collecting antenna complex II (LHCII) of photosynthetic eukaryotes is located on the thylakoid membrane of the chloroplast. It is a heterotrimer complex composed of three different subtypes of chlorophyll a/b binding proteins Lhcb1, Lhcb2 and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b binding protein in eukaryotic phototrophs and is usually encoded by multiple nuclear genes.

For research use only, not for clinical use.