Lhca5 | PSI type V chlorophyll a/b-binding protein
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Lhca5 | PSI type V chlorophyll a/b-binding protein

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Cat: PA00724
Size: 1 mg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Bryopsis corticulans, Hordeum vulgare, Spinacia oleracea
Datasheet:

Product Info

Immunogen
BSA-conjugated synthetic peptide derived from the Lhca5 protein of Arabidopsis thaliana (At1g45474). This sequence is highly conserved in Lhca5 proteins of angiosperms (monocots and dicots) and gymnosperms. UniProt: Q9C639, TAIR: At1g45474
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 200 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000-1 : 3000 (WB)
Expected | apparent MW
27.8 | 23 for Arabidopsis thaliana

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Bryopsis corticulans, Hordeum vulgare, Spinacia oleracea
Not reactive in
Chlamydomonas reinhardtii, Physcomitrella patens

Additional information

More information on the Lhca5 localization within the LHCI antenna can be found in Lucinski et al. (2006) Lhca5 interaction with plant photosystem I. FEBS Letters 580:6485-6488,Protein is processed into mature form (Jansson 1999).Urea is not recommended to use in extraction buffer and in a gel, when working with this antibody.

Description

Lhca5 is a low-content chlorophyll a/b binding protein that has been found to be subquantitatively associated with photosystem I. Unlike the four main lhca types (Lhca1-4), Lhca5 appears to be able to bind to PSI or LHCI in different locations. The expression pattern of Lhca5 gene is different from that of standard Lhc, and the expression of Lhca5 gene seems to be positively regulated under light and cold stress.

For research use only, not for clinical use.