Lhca4 | PSI type IV chlorophyll a/b-binding protein
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Lhca4 | PSI type IV chlorophyll a/b-binding protein

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Cat: PA00541
Size: 0.5 mg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Bryopsis corticulans, Citrus reticulata, Echinochloa crus-galli, Fortunella margarita Swingle, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Pisum sativum, Posidonia oceanica, Spinacia oleracea, Triticum aestivum, Triticale, Zea mays
Datasheet:

Product Info

Immunogen
BSA-conjugated synthetic peptide derived from the Lhca4 protein ofArabidopsis thaliana UniProt: P27521, TAIR: At3g47470. This sequence is highly conserved in Lhca4 proteins of angiosperms (monocots and dicots) and gymnosperms.
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2000-1 : 5000 (WB)
Expected | apparent MW
27.7 | 21 kDa for Arabidopsis thaliana

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Bryopsis corticulans, Citrus reticulata, Echinochloa crus-galli, Fortunella margarita Swingle, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Pisum sativum, Posidonia oceanica, Spinacia oleracea, Triticum aestivum, Triticale, Zea mays
Predicted reactivity
Dicots, Gymnosperms
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Protein is processed into mature form (Jansson 1999).

Description

The light harvesting protein Lhca4 is one of the four major and highly conserved chlorophyll a/b binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant light system i. Lhca4 enters the chloroplast from the cytoplasm as a precursor. After insertion into the thylakoid membrane, Lhca4 forms a heterodimer with Lhca1 (LHCI-730), which binds to the PSI core near PsaG and PsaF. Biochemical characteristics of plant LHCI antennas can be found in Klimmek et al. (2005). Structure of light harvesting complexes in higher plants I: In vivo properties and structural interdependence of Lhca proteins. Biochemistry 44:3065-3073.

For research use only, not for clinical use.