Lhca3 | PSI type III chlorophyll a/b-binding protein
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Lhca3 | PSI type III chlorophyll a/b-binding protein

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Cat: PA00540
Size: 0.5 mg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Aracis hypogae, Bryopsis corticulans, Citrus reticulata, Hordeum vulgare, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Posidonia oceanica, Spinacia oleracea, Triticum aestivum, Triticale, Zea mays
Datasheet:

Product Info

Immunogen
BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt: Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2000-1 : 5000 (WB)
Expected | apparent MW
29 | 25 kDa for Arabidopsis thaliana (due to a transit peptide being cleaved off)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Aracis hypogae, Bryopsis corticulans, Citrus reticulata, Hordeum vulgare, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Posidonia oceanica, Spinacia oleracea, Triticum aestivum, Triticale, Zea mays
Predicted reactivity
Dicots, Gymnosperms, Glycine max
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Protein is processed into mature form (Jansson 1999).

Description

The light harvesting protein Lhca3 is one of the four major and highly conserved chlorophyll a/b binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant light system i. Lhca3 enters the chloroplast from the cytoplasm as a precursor. After integration in the thylakoid membrane, Lhca3 forms a heterodimer with Lhca2 (LHCI-680), which binds to the PSI core near PsaF and PsaK. Biochemical characteristics of plant LHCI antennas can be found in Klimmek et al. (2005). Structure of light harvesting complexes in higher plants I: In vivo properties and structural interdependence of Lhca proteins. Biochemistry 44:3065-3073.

For research use only, not for clinical use.