Lens Culinaris Agglutinin (LCA), DyLight® 649
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Lens Culinaris Agglutinin (LCA), DyLight® 649

Cat: PL00697
Size: 1 mg
Sugar Specificity: Mannose
Datasheet:

Description

culinaris lectin (LCA) recognizes sequences containing alpha-linked mannose residues, but recognizes additional sugars as part of the receptor structure, making its specificity narrower than that of Con a. The α-linked focused residues attached to the n-acetyl-chitosan portion of the core oligosaccharide significantly enhance the affinity. By taking advantage of this increased specificity, after the initial separation of Con A. DyLight™649, glycoproteins and glycopeptides can be reduced with LCA labeled with the appropriate amount of binding fluorescent dye, which provides the best staining properties for this lectin. This conjugated substance basically does not contain unconjugated fluorescent dyes.

Product Info

Conjugate
DyLight 649
Concentration
1 mg active conjugate/ml
Sugar Specificity
Mannose
Storage
2-8℃
Excitation
655 nm
Emission
670 nm
Fluorescence
Far Red
Application
Immunofluorescence, Glycobiology
Recommended dilution
The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 , 0.01 mM MnCl2

For research use only, not for clinical use.