L7/L12 | Ribosomal protein

Inquiry

Cat: PA01371

Size: 200 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %

Datasheet:

Product Info

Immunogen

Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Western blot (WB)

Recommended dilution

1: 10 (IF), 1 : 1000 (WB)

Expected | apparent MW

11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)

Reactivity

Confirmed reactivity

Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul;29(1):68-88.,Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.

Description

Ribosomal protein L-30 is part of the large subunit of the ribosome 50S and is synthesized in chloroplasts. Schmidt et al.(1983) Synthesis sites of chloroplast ribosomal proteins in chlamydomonas. Chinese Journal of Biomedical Engineering, 2009 (5): 444-444

For research use only, not for clinical use.