KrP | Kelch repeat-containing protein / galactose oxidase (At3g07720)
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KrP | Kelch repeat-containing protein / galactose oxidase (At3g07720)

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Cat: PA01454
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
Recombiant KrP derived from Arabidopsis thaliana sequence, UniProt: Q9S7W4, TAIR: At3g07720
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
35,7 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

KrP (NSL1) and NSP5 (nitrile-specifier protein 5) share 53% of amino acid sequence, thus the antibody might also recognize NSP5 from different plant species,This antibody will also bind to recombinant KrP (range 10 ng - 1 µg)

Description

Kelch repeat containing protein (KrP)/ galactose oxidase is a protein associated with iron acquisition and metal homeostasis, and is a sign of iron deficiency because its transcription level is stably regulated under iron deficiency conditions. This protein can be used as a target for bHLH39, bHLH100, and bHLH101 transcription factors. The gene located at At3g07720 is considered to be the ancestor of NSP(nitrile indicator protein), NSP1, NSP2, and NSP5, unlike its progeny which exhibit nitrile indicator activity. Alias :NSL1, NSP-like1, nitrile-labeled protein-like 1

For research use only, not for clinical use.