KC1 | potassium channel KAT3

Inquiry

Cat: PA01662

Size: 200 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 50 with 125I (WB)

Expected | apparent MW

75,5 | 75 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

For detection images please, refer to the publication belowAntibody detects native and recombinant KC1,In the work of Honsbein et al, 125I has been used for detection of KC1 since this is the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)

Description

KC1 is a regulatory K+ channel subunit that is assembled with different inward correction K+ channels to influence their activity. The amount of this protein expressed is extremely low, for example only a few hundred per cell. Aliases :AKT4, AtKC1, Potassium channel TKC

For research use only, not for clinical use.