Jacalin appears to bind only o-glucosid-linked oligosaccharides, preferring galactose (β-1,3) n-acetylgalactosamine structures. This structure (t antigen) is a peanut lectin (PNA) -bound oligosaccharide. However, unlike PNA, Jacalin can bind single or double bonded forms of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also provides an opportunity to locate or isolate glycoproteins with oligosaccharide side chains that have O-glycoside connections. Fluorescein-labeled Jacalin has the right amount of fluorescent dye binding to provide the best staining properties for this lectin. This conjugated substance basically does not contain unconjugated fluorescent dyes. The excitation maximum is 495nm and the emission maximum is 515nm.
For research use only, not for clinical use.