Jacalin, Fluorescein
Products
Online Inquiry

Jacalin, Fluorescein

Cat: PL00678
Size: 5 mg
Sugar Specificity: Galactose
Datasheet:

Description

Jacalin appears to bind only o-glucosid-linked oligosaccharides, preferring galactose (β-1,3) n-acetylgalactosamine structures. This structure (t antigen) is a peanut lectin (PNA) -bound oligosaccharide. However, unlike PNA, Jacalin can bind single or double bonded forms of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also provides an opportunity to locate or isolate glycoproteins with oligosaccharide side chains that have O-glycoside connections. Fluorescein-labeled Jacalin has the right amount of fluorescent dye binding to provide the best staining properties for this lectin. This conjugated substance basically does not contain unconjugated fluorescent dyes. The excitation maximum is 495nm and the emission maximum is 515nm.

Product Info

Conjugate
Fluorescein
Concentration
5 mg active conjugate/ml
Sugar Specificity
Galactose
Storage
2-8℃
Excitation
495-500 nm
Emission
514-521 nm
Fluorescence
Green
Application
Immunofluorescence, Glycobiology
Recommended dilution
The recommended concentration range for use is 5-20 µg/ml.
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 10 mM lactose, 10 mM Beta-Cyclodextrin

For research use only, not for clinical use.