Jacalin, Agarose bound

Inquiry

Cat: PL00661

Size: 10 ml

Sugar Specificity: Galactose

Datasheet:

Description

Agarose binding Jacalin is prepared with our affinity purified lectins. Jacalin is a lectin composed of four subunits, each about 16 kDa. This lectin appears to bind only O-glucosid-linked oligosaccharides, preferring the structure of galactose (β-1,3) n-acetylgalactosamine. This structure (t antigen) is a peanut lectin (PNA) -bound oligosaccharide. Unlike PNA, however, Jacalin will bind to a single-stranded or dealkylated form of the structure. This lectin has been used to purify human IgA. The specificity of this lectin also provides an opportunity to locate or isolate glycoproteins with oligosaccharide side chains that have O-glycoside connections.

Product Info

Conjugate

Agarose

Sugar Specificity

Galactose

Storage

2-8℃

Application

Glycobiology, Affinity Chromatography

Recommended dilution

Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Note:To optimize elution of glycoproteins, wash the gel with 10 column volumes of 175 mM TRIS, pH 7.5, before use.Glycoproteins should be applied in this buffer. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 0.1 M melibiose or 0.8 M galactose in 175 mM TRIS, pH 7.5 can be used.Use of other buffers may result in a reduction in yield of eluted glycoproteins. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.

Solution

10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 20 mM lactose, 0.08% sodium azide

For research use only, not for clinical use.