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Isoamylase 1, chloroplastic (ISA1), Recombinant Protein
Cat: RP01208
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Isoamylase 1, chloroplastic (ISA1) , partial
Product Gene Name
ISA1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
89,481 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Isoamylase
NCBI/Uniprot Data
NCBI Accession #
NP_181522.1
NCBI GI #
15225595
NCBI GenBank Nucleotide #
NM_129551.4
NCBI GeneID
818580
NCBI Official Full Name
isoamylase 1
NCBI Official Symbol
ISA1
NCBI Official Synonym Symbols
ARABIDOPSIS THALIANA ISOAMYLASE 1; ATISA1; isoamylase 1; T28M21.9; T28M21_9
NCBI Protein Information
isoamylase 1
NCBI Summary
Encodes an isoamylase-type debranching enzyme. Mutations in this gene cause the loss of detectable isoamylase activity and the disruption of normal starch structure. Mutants have reduced starch content and abnormally structured amylopectins and phytoglycogens. It has been postulated that AtISA1 interacts with AtISA2 to form the Iso1 complex.
UniProt Gene Name
ISA1
UniProt Synonym Gene Names
AtISA1
UniProt Protein Name
Isoamylase 1, chloroplastic
UniProt Primary Accession #
O04196
UniProt Related Accession #
O04196
UniProt Comments
Involved in the trimming of pre-amylopectin chains. Accelerates the crystallization of nascent amylopectin molecules during starch synthesis. ISA1 and ISA2 work exclusively together as a multimeric holoenzyme. ISA1-ISA2 removes preferentially branches that are very close to other branches.
For research use only, not for clinical use.