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Iron-sulfur cluster assembly protein 3 (ISU3), Recombinant Protein
Cat: RP02120
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Iron-sulfur cluster assembly protein 3 (ISU3)
Product Gene Name
ISU3 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
PNVGTGLVGA PQCGDVMKLQ VKFDGSGQII DAKFKTFGCG SAIAASSVAT EWVKGKSVEE VLTIKNSQIA KHLSLPPVKL HCSMLAEDAI KAAIKNYKEK QDKANGETVE TIDSTYLHGI GS
Sequence Positions
50-171, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
18,471 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Iron-sulfur cluster assembly protein
NCBI/Uniprot Data
NCBI Accession #
NP_192317.1
NCBI GI #
15233385
NCBI GenBank Nucleotide #
NM_116646.3
NCBI GeneID
825719
NCBI Official Full Name
ISCU-like 3
NCBI Official Symbol
ISU3
NCBI Official Synonym Symbols
ATISU3; ISCU-like 3; ISCU-LIKE 3; T24H24.11; T24H24_11
NCBI Protein Information
ISCU-like 3
NCBI Summary
Encodes a mitochondrial protein similar to E.coli IscU. In bacteria, IscU is a scaffold protein accepting sulfur and iron to build a transient Fe-S cluster,which is subsequently transferred to a target apoprotein.
UniProt Gene Name
ISU3
UniProt Synonym Gene Names
AtISU3; Protein ISCU-LIKE 3
UniProt Protein Name
Iron-sulfur cluster assembly protein 3
UniProt Synonym Protein Names
NifU-like N-terminal domain-containing protein ISU3; NifU-like protein ISU3
UniProt Primary Accession #
O81433
UniProt Related Accession #
O81433
UniProt Comments
Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:17417719). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU (ISU1, ISU2 or ISU3). In a second step, the cluster is released from ISCU, transferred to a glutaredoxin, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH .
For research use only, not for clinical use.