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Iron-sulfur cluster assembly protein 1 (ISU1), Recombinant Protein
Cat: RP01861
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Iron-sulfur cluster assembly protein 1 (ISU1)
Product Gene Name
ISU1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
PNVGTGLVGA PACGDVMKLQ IKVDEKTGQI VDARFKTFGC GSAIASSSVA TEWVKGKAME DVLTIKNTEI AKHLSLPPVK LHCSMLAEDA IKAAVKDYKE KRVKTNGAAA AGETTQA
Sequence Positions
51-167, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
17,915 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Iron sulfur cluster assembly protein
NCBI/Uniprot Data
NCBI Accession #
NP_193953.1
NCBI GI #
15235569
NCBI GenBank Nucleotide #
NM_118347.3
NCBI GeneID
828316
NCBI Official Full Name
SufE/NifU family protein
NCBI Official Symbol
ISU1
NCBI Official Synonym Symbols
ATISU1; T10I14.50; T10I14_50
NCBI Protein Information
SufE/NifU family protein
NCBI Summary
Encodes a mitochondrial protein similar to E.coli IscU. In bacteria, IscU is a scaffold protein accepting sulfur and iron to build a transient Fe-S cluster,which is subsequently transferred to a target apoprotein.
UniProt Gene Name
ISU1
UniProt Synonym Gene Names
AtISU1; Protein ISCU-LIKE 1
UniProt Protein Name
Iron-sulfur cluster assembly protein 1
UniProt Synonym Protein Names
NifU-like N-terminal domain-containing protein ISU1; NifU-like protein ISU1
UniProt Primary Accession #
O49627
UniProt Secondary Accession #
Q8L984
UniProt Related Accession #
O49627
UniProt Comments
Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:15507320, PubMed:17417719). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU (ISU1, ISU2 or ISU3). In a second step, the cluster is released from ISCU, transferred to a glutaredoxin, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH .
For research use only, not for clinical use.