Immobilized Papain

Format

Mixture

Storage

2-8°C

Additional information

Ensure good ventilation of the work station. Avoid release to the environment.

Application

Immobilized Papain is a convenient reagent for producing Fab and Fc fragments as it avoids the need to remove the papain enzyme after digestion.

Description

Papain is a cysteine protease (EC 3.4.22.2) with endopeptidase activity for cutting immunoglobulin G molecules in the hinge region. 3 ~50kDa fragments were produced by pyrolysis. Two Fab domains and one Fc domain. Papain digested antibodies do not promote agglutination, precipitation, conditioning, and cleavage. Fab and Fc fragments can be further separated by immobilized protein A or ion exchange chromatography.