Immobilized Ficin

Format

Mixture

Storage

Store in the original container in a dry, cool and well-ventilated area away from direct sunlight between the following temperatures: 2 to 8°C.

Additional information

Avoid release to the environment. The product is non-reactive under normal conditions of use, storage and transport.

Application

Immobilized Ficin is a convenient reagent for producing Fab and F(ab′)2 fragments as it avoids the need to remove the ficin enzyme after digestion.

Description

Ficin is a cysteine protease isolated from FIG latex (EC 3.4.22.3) with endopeptidase activity for cutting immunoglobulin G molecules in the hinge. Ficin is commonly used to cut mouse IgG1, as this is difficult to cut with papain and pepsin. In the presence of 1mM or 10mM cysteine, ficin produces F(ab ')2 and Fab fragments, respectively. Fab and F(ab ')2 fragments can be isolated from whole IgG and Fc using immobilized protein A or ion exchange chromatography.