IDH | Isocitrate dehydrogenase (Cellular [compartment marker] of mitochondrial matrix)

Cat: PA00360

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Lycopersicum chilense, Nicotiana benthamiana, Oryza sativa, Solanum lycopersicum, Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial, NAD dependent isocitrate dehydrogenase subunits including Arabidopsis thaliana IDH-I Q8LFC0, At4g35260 and IDH-II P93032, At2g17130

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Western blot (WB)

Recommended dilution

1:400 (IF), 1 : 5 000 (WB)

Expected | apparent MW

39 | 45 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Lycopersicum chilense, Nicotiana benthamiana, Oryza sativa, Solanum lycopersicum, Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays

Predicted reactivity

Brachypodium distachyon, Brassica napus, Capsella rubella, Citrus sinensis, Glycine max, Hordeum vulgare, Malus x domestica, Medicago truncatula, Nicotiana tabacum, Phaseolus vulgaris, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays

Not reactive in

Chlamydomonas reinhardtii

Additional information

Peptide used to elicit this antibody is not conserved in NADPH dependent anzymes, partially conserved across eukaryotic Idh subunits, Some conservation across bacterial which contain the NAD-dependent form of Idh (as opposed to the NADP-dependent form),Cellular [compartment marker] of mitochondrial matrix

Description

Plant NADH-dependent isocitrate dehydrogenase is located in the mitochondrial matrix. This enzyme is classified as a oxidoreductase and its function is to catalyze reactions in the citric acid cycle, in particular the continuous dehydrogenation and decarboxylation of isocitrate to form A-ketoglutaric acid. It removes hydrogen from the substrate isocitrate. In addition to this process, it also acts as a decarboxylase, removing carbon dioxide from the six-carbon substrate to form the aforementioned five-carbon product A-ketoglutarate. This enzyme comes in two forms: NADP+ dependent and NAD+ dependent.

For research use only, not for clinical use.