ICE1 | Inducer of CBF expression 1

Inquiry

Cat: PA01551

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ICE1 sequence, UniProt: Q9LSE2#Q9LSE2-2, TAIR: AT3G26744. The peptide is not conserved in protein coded by SCRM2 gene. UniProt: A0A1P8AMV9

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

43 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Brassica napus, Brassica rapa, Capsella bursa-pastoris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens

Not reactive in

Solanum lycopersicum

Description

ICE1 (Inducer of CBF expression 1) is a negative regulator of brassinolide signal transduction pathway and is important for plant growth. Phosphorylation and increased proteasome degradation of BZR1 and BZR2/BES1. BHLH150, beet roll top virus C4 and tomato Mosaic virus AC4 are phosphorylated on threonine and serine residues. Based on brassinolide signaling, stomatal development is inhibited by phosphorylation and inhibition of MAPKK kinase YDA and MAPK kinase MKK4 and MKK5.

For research use only, not for clinical use.