Total IgG is purified by ammonium sulfate precipiation,For detailed immunolocalization protocol check here. Quantification of IAA in ELISA using this antibody is described in Bianco and Defez (2009).Whole-mount immunolocalization is described in Lu et al. (2015).
Indoleacetic acid (IAA) is the main growth hormone of higher plants. This hormone is produced in the cells at the tip of the plant and in young leaves. Plant cells synthesize IAA from tryptophan. The different effects of auxin include: inducing cell elongation and cell division, and subsequent effects on plant growth and development.
For research use only, not for clinical use.