IAA-amino acid hydrolase ILR1 (ILR1), Recombinant Protein
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IAA-amino acid hydrolase ILR1 (ILR1), Recombinant Protein

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Cat: RP00893
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana IAA-amino acid hydrolase ILR1 (ILR1)
Product Gene Name
ILR1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
GSYDSGSGLE SLARGMLHSA KDPEFFEWMR GIRRKIHENP ETGFQEFKTS QLVRDELDSL GVKYKYPVAK TGVVAWIGSC SKPVFGLRAD MDALPLQELV EWESKSKVDG KMHACGHDTH VAMLLGAAKL LQTTKHLIKG TVKLVFQPGE EGYAGAYEML KDEILDDLDG ILSVHVFPSI PSGGIGSRPG TVLAGAGLFT VTVHGQGSHA ATPHFSKDPV LAASSAVVAL QQIVSRELDP LEAGVVTVGY IEGGHAQNVI PQSAKFGGTF RSLSNDGLLF IQRRIKEISE AQASVYRCKA EVNFEEKKPS LHPVMNNDEG LYEHGKKVAE AMIGKNNFHD FPVTMGGEDF SFFTQKTKAA IFVLGVKNET LGAGKPLHSP YFFVDEEALP VGAALHAAMA VSYLDEHGHS HEEEVKSEL
Sequence Positions
24-442, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
48,015 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
IAA-amino acid hydrolase

NCBI/Uniprot Data

NCBI Accession #
NP_186937.1
NCBI GI #
15233011
NCBI GenBank Nucleotide #
NM_111156.3
NCBI GeneID
821199
NCBI Official Full Name
Peptidase M20/M25/M40 family protein
NCBI Official Symbol
ILR1
NCBI Official Synonym Symbols
F13E7.18; F13E7_18; IAA-AMINO ACID HYDROLASE; IAA-LEUCINE RESISTANT 1
NCBI Protein Information
Peptidase M20/M25/M40 family protein
NCBI Summary
Hydrolyzes amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Leu and IAA-Phe. Uses Mg and Co ions as cofactors.
UniProt Gene Name
ILR1
UniProt Protein Name
IAA-amino acid hydrolase ILR1
UniProt Primary Accession #
P54968
UniProt Secondary Accession #
Q9M8S9
UniProt Related Accession #
P54968
UniProt Comments
Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Phe, IAA-Leu and IAA-Tyr (PubMed:7792599, PubMed:10072397, PubMed:11923288). Can also use IAA-Ala, IAA-Gly, IAA-Met and IAA-Glu as substrates with low efficiency (PubMed:7792599, PubMed:10072397, PubMed:11923288). No activity with IAA-Ile, IAA-1-O-beta-D-glucose or IAA-myo-inositol (PubMed:7792599, PubMed:10072397, PubMed:11923288). Is the most efficient enzyme of the ILL family for IAA-Leu hydrolysis (PubMed:11923288). Important for IAA-Leu and IAA-Phe hydrolysis in roots (PubMed:15155875). May act with ILL2 to provide free IAA to germinating seedlings (PubMed:15155875).

For research use only, not for clinical use.