HSP70 | Heat shock protein 70 (cytoplasmic)

Cat: PA00972

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Brassica juncea, Brassica napus, Brassica oleracea, Chlamydomonas reinhardtii, Chlamydomonas sp. UWO241, Cucumis sativus, E. teft, Fagopyrum esculentum, Hordeum vulgare, Medicago sativa, Oryza sativa, Salicornia sp., Silene vulgaris, Solanum lycopersicum, Picrorhiza kurroa, Pinus strobus, Polyscias elegans, Zea mays, algae: Desmodesmus subspicatus, Gracilaria vermiculophylla, phycobiont: Trebouxia TR1 and TR9, Plasmodium falciparum, Setaria italica, Triticum aestivum, Ulva prolifera, Vicia faba

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 UniProt: F4KCE5 , HSC70-2 UniProt: A0A178UTH3 and HSC70-3 Uniprot: O65719 as well as heat shock inducible Hsp70 of Arabidopsis thaliana TAIR: AT3g12580/T2E22_110 and At1g16030 and AT3g12580/T2E22_110

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1 : 3000 - 1: 10 000, 5 µg protein/well (WB), 2-3 µl/protein extract of concentration 3-5 mg/ml

Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Brassica juncea, Brassica napus, Brassica oleracea, Chlamydomonas reinhardtii, Chlamydomonas sp. UWO241, Cucumis sativus, E. teft, Fagopyrum esculentum, Hordeum vulgare, Medicago sativa, Oryza sativa, Salicornia sp., Silene vulgaris, Solanum lycopersicum, Picrorhiza kurroa, Pinus strobus, Polyscias elegans, Zea mays, algae: Desmodesmus subspicatus, Gracilaria vermiculophylla, phycobiont: Trebouxia TR1 and TR9, Plasmodium falciparum, Setaria italica, Triticum aestivum, Ulva prolifera, Vicia faba

Predicted reactivity

Ageratina adenophora, Allium sativum, Arabis alpina, Arachis diogoi, Arundo donax, Brassica napus, brassica rapa subsp. pekinensis, Camellia sinensis, Citrus sinensis, Coffea arabica, Eriobotrya japonica, Gossypium arboretum, Glycine max, Glycine soja, Helianthus annuus, Hordeum vulgare var. distichum, Lotus japonicus, Medicago sativa, medicago truncatula, Musa acuminata subsp. malaccensis, Nannochloropsis gaditana, Nicotiana tabacum, Nicotiana bethamiana, Olea europea, Phaseolus vulgaris, Physcomitrium patens, Pinus taeda, Pisum sativum, Populus balsamifera, Populus trichocarpa, Salix gilgiana, Saussurea medusa,Solanum tuberosum, Solanum commersonii, Spinacia oleracea, Tragopogon dubius, Tragopogon porrifolius, Triticum aestivum, Vitis vinifera, Volvox sp.

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This product can be sold containing ProClin if requested,Can be sold containing 0.1% ProClin if requested This antibody can be used as a marker of cytoplasmic fraction in tomato (Anfoka et al. 2015).Applied primary antibody dilution in western blot depends upon sensitivity of detection reagents (pico or femtogram for chemiluminescent detection).Immunoprecipitation protocol using Agrisera anti-Hsp70 cytosolic antibodies, see tab: protocols.

Description

Heat shock protein 70 (Hsp70) is a major stress-inducing protein in vertebrates and has been highly conserved throughout evolution. It acts as a molecular chaperone and is important for allowing cells to respond to acute stressor damage, particularly those that affect protein mechanisms. Heat shock homologous protein 70 (HSC70) is a highly conserved protein that is a member of the chaperone family of proteins.

For research use only, not for clinical use.