HSP26,5 | Heat shock protein 26,5 (mitochondrial)

Inquiry

Cat: PA01445

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana HSP26.5, mitochondrial, UniProt: Q9SSQ8, TAIR: At1g52560

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 25 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

26,5 | 23 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Glycine soja

Additional information

Detection on total extracts needs to be optimized.Antibody is recognizing HSP23.6 synthesized in vitro using the PURExpress in Vitro Protein Synthesis Kt (NEB).,Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C. Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.

Description

HSP26.5 | Heat shock protein 26.5(mitochondria) belongs to a small family of heat shock proteins located in the mitochondria.

For research use only, not for clinical use.