Detection on total extracts needs to be optimized.Antibody is recognizing HSP23.6 synthesized in vitro using the PURExpress in Vitro Protein Synthesis Kt (NEB).Antibody is not cross reacting with cytosolic HSPs.,Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C. Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.
HSP23.6(Heat shock protein 23.6(mitochondria)) belongs to a small family of heat shock proteins that are localized in mitochondria. For short :AtHsp23.6.
For research use only, not for clinical use.