HSP101 | ClpB heat shock protein, C-terminal

When using this antibody on a new species with this antibody perform a following experiment: prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38°C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench). You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample. Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.

Hsp101/ClpB is a member of the HSP100 protein family. These proteins help protein aggregates formed during heat stress break down, allowing them to be refolded by other companion proteins. In addition to being expressed during heat stress, members of the HSP100 protein family are also expressed during seed development.