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His-tag | 3xHis (polyclonal)
Cat: PA00088
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: 3xHis tagged proteins
Datasheet:
Product Info
Immunogen
KLH-conjugated synthetic peptide 3xHis
Host
Rabbit
Clonality
Polyclonal
Purity
Antigen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 μg/mL (IF), 1: 1000 - 1 : 5000 (WB)
Expected | apparent MW
3xHis tagged proteins
Reactivity
Confirmed reactivity
3xHis tagged proteins
Description
His-Tag is a multi-histidine tag that is introduced by 3 or 6 histidine residues to the N or c terminal of a protein. The multi-histidine label can be used for recombinant protein detection using specific antibodies that do not bind to any dye or enzyme.
For research use only, not for clinical use.