H+ATPase | Plasma membrane H+ATPase (chicken antibody)

Inquiry

Cat: PA00526

Size: 50 µg

Host: Chicken

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Spinacia oleracea, Zea mays

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Host

Chicken

Clonality

Polyclonal

Purity

Immunogen affinity purified IgY in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000-1 : 5000 (WB)

Expected | apparent MW

95 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Spinacia oleracea, Zea mays

Predicted reactivity

Angomonas deanei, Avena sativa, Brassica napus, Citrus limon, Coffea canephora, Cucumis sativus, Cucurbita moschata, Dunaliella spp, Eichhornia crassipes, Emiliana huxleyi, Glycine max (weak), Hordeum vulgare, Lactobacillus johnsonii, Laishamania braziliensis, Nicotiana tabacum, Oryza sativa, Solanum lycopersicon, Solanum tuberosum, Medicago truncatula, Mesembruanthemum crystallinum), Nannochloropsis gaditana CCMP526, Nepenthes alata, Nicotiana tabaccum, Nitrospira bacterium, Oryza sativa, Ostreococcus spp., Phaseolus acutifolius, Physocomitrella patens, Picea abies, Pinus thunbergii, Populus tremula, Pteris vittata, Ricinus communis, Saccharomyces cerevisiae, Solanum lycopersicum, Strigomonas culicis, Toxoplasma gondii, Triticum urartu, Trypanosoma cruzi, Zosteria marina, Vicia faba, Vigna angularis

Additional information

Cellular [compartment marker] for plasma membranetissue specific immunolocalization is done on paraffin emdedded samples as described here,VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot

Description

Plasma membrane H+ ATPase is a family of approximately 100 kDa proteins thought to be found only in the plasma membranes of plants and fungi. Proteins are fixed within the biofilm, creating an electrochemical gradient that acts as an energy source and is essential for the absorption of most metabolites and for the plant's response to the environment, such as leaf movement.

For research use only, not for clinical use.