H3T6pK9me1 | Histone H3 methylated at Lys9 (K9)

Inquiry

Cat: PA01325

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Caenorhabditis elegans, Human

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum.

Format

Liquid

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution

2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 100 (IF), 1 : 200 (IHC), 1 : 500-1 : 1000 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity

Caenorhabditis elegans, Human

Predicted reactivity

Chicken, Drosophila melanogaster, Human, Mouse, Plant, Rat, Xenopus sp.

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol

Description

Methylation of histone H3 at Lys9 (K9) is the epigenetic silencer of transcription. Gene silencing by posttranslational modification of histones, as well as DNA methylation, plays a key role in the development of normal tissues.

For research use only, not for clinical use.