H3T3pK4ac | Histone H3 (ac Lys4, p Thr3)
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H3T3pK4ac | Histone H3 (ac Lys4, p Thr3)

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Cat: PA01321
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Caenorhabditis elegans, Human
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic acetylated/phosphorylated peptide surrounding Lysine 4 and Threonine 3 of human Histone H3
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum.
Format
Liquid
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 100 (IF), 1 : 50 (IHC), 1 : 500 (WB)
Expected | apparent MW
15 kDa

Reactivity

Confirmed reactivity
Caenorhabditis elegans, Human
Predicted reactivity
Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol

Description

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Chromatin, in turn, is formed from nucleosomes, which consist of a set of four histones (H2A, H2B, H3, H4) wrapped around DNA. Chromatin is a very dynamic structure in which many post-translational modifications work together to activate or inhibit the replication, transcription, or repair availability of DNA. These markers determine which DNA is open and usually active (euchromatin) and which is tightly wound to prevent entry and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, threonylation of lysine, biotinylation, and ubiquitination. Phosphorylation of threonine 3 (H3T3p), a known mitotic marker, is modified by the enzyme Haspin/Thr3, while acetylation of lysine 4 (H3K4ac) on histone 3 is associated with transcriptional activation of Esa1. Alternative names: H3.3b, H3 histone, Family 3A, H3.3AH3F3H3F3b, Histone H3.3, MGC87782, MGC87783, H3pT3/K4ac.

For research use only, not for clinical use.