H3S10pT11p | Histone H3 (p Ser10, p Thr11)

This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol

When faced with DNA damage or the absence of certain kinases, such as Chk1 and Aurora1, histone H3 is phosphorylated at T11, resulting in reduced acetylation of the same histone at K9, ultimately leading to less active transcription in the affected cells. When T11 phosphorylation binds to typical S10 phosphorylation, which plays a key role in metaphase chromosome aggregation, the effect on transcriptional regulation is further amplified. Phosphorylation of H3S10 is reduced by phosphorylation of T11. Therefore, detection of combined modifications is critical for determining transcriptional effects, especially in comparison to single modifications of H3.