H3K4me3 | Histone H3, trimethylated lysine 4 (H3K4me3)

Inquiry

Cat: PA00825

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Human, Solanum lycopersicum

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide

Host

Rabbit

Clonality

Polyclonal

Purity

Antigen affinity purified

Format

Liquid

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)

Recommended dilution

1-5 µg/IP (ChIP-seq), 1 : 10 000 (Dot), 1 : 100 (ELISA), 1 : 200 (IF), 1 : 2000 (PA), 1 : 1000 (WB)

Reactivity

Confirmed reactivity

Human, Solanum lycopersicum

Predicted reactivity

Arabidopsis thaliana, Mouse, Oryza sativa, Populus sp., Zea mays

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300 at concentration of 1.3 µg/µl.

Description

Histone is the main component of chromosome protein in eukaryotic cells. They are rich in the amino acids arginine and lysine, which have been highly conserved during evolution. Histones wrap DNA into tight clumps of chromatin. The two core histones of each class H2A, H2B, H3 and H4 are assembled and wrapped by 146 base pairs of DNA to form an octamer nucleosome. Histone tails undergo many post-translational modifications that directly or indirectly alter chromatin structure, promote transcriptional activation or inhibition, or other nuclear processes. Methylation of histone H3K4 is associated with activation of gene transcription.

For research use only, not for clinical use.