H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)

Inquiry

Cat: PA01305

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Human

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum.

Format

Liquid

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)

Recommended dilution

0,5-5 µg/IP (ChIP-seq), 1 : 20 000 (Dot), 1 : 500 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity

Human

Predicted reactivity

Arabidopsis thaliana, Mouse, Rat

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300,Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300

Description

Histone methylation and demethylation are dynamically regulated by histone methyltransferase and histone demethylase, respectively. Acetylation of histone H3K27 is associated with active promoters and enhancers.

For research use only, not for clinical use.