H3K18me2 | Histone H3 (dimethylated Lys18)
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H3K18me2 | Histone H3 (dimethylated Lys18)

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Cat: PA01303
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Human
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum.
Format
Liquid
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 500 (IF), 1 : 100 (IHC), 1 : 500 (WB)
Expected | apparent MW
15 kDa

Reactivity

Confirmed reactivity
Human
Predicted reactivity
Caenorhabditis elegans, Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol

Description

Dimethylation K18 on histone H3 appears to be a transient posttranslational modification. H3K18 is known to be acetylated, and occasionally monomethylation occurs. Suv39h1, a well-studied histone methyltransferase, appears to be responsible for the transition of acetylation and methylation at this H3 modification site. Dimethylation K18 on H3 appears to be associated with embryonic development and possible implantation.

For research use only, not for clinical use.