H3 | Histone H3 (rabbit antibody) (nuclear marker)
  1. Home
  2. Products
  3. Plant Antibodies
  4. Plant General Antibodies
  5. Loading Controls
Products
Online Inquiry

H3 | Histone H3 (rabbit antibody) (nuclear marker)

Inquiry
Cat: PA01003
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, Cucurbita pepo L. var. cylindrica, Hordeum vulgare, human, Nicotiana benthamiana, Phalaenopsis equestris, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030, At4g40040, At5g10980), H3.2 P59226 (At1g09200, At3g27360, At5g10390, At5g10400, At5g65360), H3-like 2 Q9FXI7 (At1g19890)
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
ChIp-qPCR (ChIp-qPCR), Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 100-1 : 500 (ICC), 2 µl of antibody/500 µl solution (ChIp-qPCR), 1: 500 (IF),  1 : 5000 (WB)
Expected | apparent MW
15 | 17 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, Cucurbita pepo L. var. cylindrica, Hordeum vulgare, human, Nicotiana benthamiana, Phalaenopsis equestris, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays
Predicted reactivity
Brachypodium distachyon, Brassica napus, Emiliania huxleyi, Glycine max, Nannochloropsis gaditana, Nicotiana tabacum, Malus domestica, Medicago sativa, Oryza sativa, Ostreococcus sp. , Pinus pinaster, Pisum sativum, Porphyridium purpureum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox sp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii,Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes

Description

Histone 3 (H3) is located in the nucleus and incorporated into chromatin. It is present in nucleosomes along with H2A, H2B, and H4.

For research use only, not for clinical use.