Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Biotinylated
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Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Biotinylated

Cat: PL00691
Size: 2 mg
Sugar Specificity: Galactose, N-Acetylgalactosamine
Datasheet:

Description

GSLI is a family of glycoproteins with a molecular weight of about 114 kDa. There are two types of subunits, called "A" and "B", with slightly different molecular weights. These subunits combine to form a tetramer structure, producing five isolates. A-rich lectins preferentially agglutinate group A red blood cells and therefore appear to be specific to α-n-acetylgalactosamine residues, while B-rich lectins preferentially agglutinate group B cells and are specific to α-galactosamine residues. Our GSLI is a mixture of five isolates. GSLI has been reported to bind to a variety of glycoproteins, including laminin. Biotinylated GSLI combines the appropriate amount of biotin to provide optimal staining properties for this lectin.

Product Info

Conjugate
Biotinylated
Concentration
2 mg active conjugate/ml
Sugar Specificity
Galactose, N-Acetylgalactosamine
Storage
2-8℃; Store frozen for long term storage
Application
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs
Recommended dilution
For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.

For research use only, not for clinical use.